Journal: Virology Journal
Article Title: Tropism of adeno-associated virus serotypes in mouse lungs via intratracheal instillation
doi: 10.1186/s12985-024-02575-9
Figure Lengend Snippet: Quantification of pulmonary epithelial cells that are infected by AAVs. a Ratio of SCGB1A1 + cells that are eGFP + . 1754, 260, 0, 0, 94, 1308, 0, 1282, 1240, 1470, 74, 126, 0, 0, 126, 254, 0, 276, 366, 0 for eGFP + SCGB1A1 + cells were analyzed for AAV1, AAV2, MyoAAV 2A, AAV2-retro, AAV3b, AAV4, MyoAAV 4A, AAV5, AAV6, AAV6.2, AAV7m8, AAV8, AAV9, AAVrh10, AAV-DJ, AAV-DJ/8, AAVie, AAV-PHP.B, AAV-PHP.eB, AAV-PHP.S, and 2456, 3116, 2478, 2856, 2094, 1904, 2226, 1904, 1586, 1932, 1724, 1940, 1204, 1372, 2824, 2996, 1778, 2300, 2460, 1736 for SCGB1A1 + cells, respectively. n = 3. b Ratio of RFX3 + cells that are eGFP + . 612, 0, 0, 0, 0, 496, 0, 802, 448, 634, 178, 0, 0, 0, 0, 176, 0, 712, 0, 0 for RFX3 + eGFP + cells were analyzed for AAV1, AAV2, MyoAAV 2A, AAV2-retro, AAV3b, AAV4, MyoAAV 4A, AAV5, AAV6, AAV6.2, AAV7m8, AAV8, AAV9, AAVrh10, AAV-DJ, AAV-DJ/8, AAVie, AAV-PHP.B, AAV-PHP.eB, AAV-PHP.S, and 3838, 3808, 5224, 5976, 6526, 2280, 4572, 2978, 1818, 2328, 3848, 2734, 2602, 2800, 4156, 5136, 3778, 4634, 4940, 4376 for RFX3 + cells, respectively. n = 3. c Ratio of Acetyl-α-Tubulin + cells that are eGFP + .778, 0, 0, 0, 0, 642, 0, 822, 562, 666, 174, 0, 0, 0, 0, 178, 0, 616, 0, 0 for Acetyl-α-Tubulin + eGFP + cells were analyzed for AAV1, AAV2, MyoAAV 2A, AAV2-retro, AAV3b, AAV4, MyoAAV 4A, AAV5, AAV6, AAV6.2, AAV7m8, AAV8, AAV9, AAVrh10, AAV-DJ, AAV-DJ/8, AAVie, AAV-PHP.B, AAV-PHP.eB, AAV-PHP.S, and 4704, 4590, 6018, 6834, 8024, 2930, 5508, 3204, 2320, 2634, 3974, 3672, 5916, 5032, 5270, 5780, 4216, 4090, 4046, 5678 for Acetyl-α-Tubulin + cells, respectively. n = 3. d Ratio of HOPX + cells that are eGFP + . 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 78, 0, 132, 0, 0, 0, 0, 0, 0 for HOPX + eGFP + cells were analyzed for AAV1, AAV2, MyoAAV 2A, AAV2-retro, AAV3b, AAV4, MyoAAV 4A, AAV5, AAV6, AAV6.2, AAV7m8, AAV8, AAV9, AAVrh10, AAV-DJ, AAV-DJ/8, AAVie, AAV-PHP.B, AAV-PHP.eB, AAV-PHP.S, and 1470, 3780, 2478, 3136, 3234, 2828, 2226, 4074, 2996, 3052, 3780, 2898, 2772, 3606, 3262, 2142, 1778, 1148, 1890, 1302 for HOPX + cells, respectively. n = 3. e Ratio of SFTPC + cells that are eGFP + . 1020, 408, 0, 252, 444, 766, 0, 2242, 2534, 2330, 1668, 1512, 1054, 1040, 950, 1144, 2142, 662, 860, 490 for SFTPC + eGFP + cells were analyzed for AAV1, AAV2, MyoAAV 2A, AAV2-retro, AAV3b, AAV4, MyoAAV 4A, AAV5, AAV6, AAV6.2, AAV7m8, AAV8, AAV9, AAVrh10, AAV-DJ, AAV-DJ/8, AAVie, AAV-PHP.B, AAV-PHP.eB, AAV-PHP.S, and 1586, 3782, 2478, 4250, 6468, 1568, 2226, 4070, 3582, 3348, 9194, 5282, 5366, 5340, 3070, 3260, 3728, 2436, 2600, 2120 for SFTPC + cells, respectively. n = 3. f Ratio of ERG + cells that are eGFP + . 66, 58, 0, 72, 0, 52, 0, 96, 98, 76, 0, 78, 0, 0, 76, 76, 114, 122, 64, 70 for ERG + eGFP + cells were analyzed for AAV1, AAV2, MyoAAV 2A, AAV2-retro, AAV3b, AAV4, MyoAAV 4A, AAV5, AAV6, AAV6.2, AAV7m8, AAV8, AAV9, AAVrh10, AAV-DJ, AAV-DJ/8, AAVie, AAV-PHP.B, AAV-PHP.eB, AAV-PHP.S, and 8364, 19,142, 10,392, 22,746, 13,582, 12,748, 9490, 16,240, 26,052, 19,534, 11,782, 20,028, 8488, 8262, 16,880, 14,944, 15,482, 11,352, 10,870, 9828 for ERG + cells, respectively. n = 3
Article Snippet: AAVs serotypes (PackGene Biotech) used in this study were as follows: AAV1-CAG-Dio-eGFP-WPRE-pA, AAV2-CAG-Dio-eGFP-WPRE-pA, MyoAAV 2A-CAG-Dio-eGFP-WPRE-pA, AAV2-retro-CAG-Dio-eGFP-WPRE-pA, AAV3b-CAG-Dio-eGFP-WPRE-pA, AAV4-CAG-Dio-eGFP-WPRE-pA, MyoAAV 4A-CAG-Dio-eGFP-WPRE-pA, AAV5-CAG-Dio-eGFP-WPRE-pA, AAV6-CAG-Dio-eGFP-WPRE-pA, AAV6.2-CAG-Dio-eGFP-WPRE-pA, AAV7m8-CAG-Dio-eGFP-WPRE-pA, AAV8-CAG-Dio-eGFP-WPRE-pA, AAV9-CAG-Dio-eGFP-WPRE-pA, AAVrh10-CAG-Dio-eGFP-WPRE-pA, AAV-DJ-CAG-Dio-eGFP-WPRE-pA, AAV-DJ/8-CAG-Dio-eGFP-WPRE-pA, AAV-ie-CAG-Dio-eGFP-WPRE-pA, AAV-PHP.B-CAG-Dio-eGFP-WPRE-pA, AAV-PHP.eB-CAG-Dio-eGFP-WPRE-pA, and AAV-PHP.S-CAG-Dio-eGFP-WPRE-pA. For each mouse, 40 μL of AAV-containing solution (10 [ ] GC/mL) was injected into the lungs through the trachea.
Techniques: Infection
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